Posts Tagged ‘Techniques’
Last Updated on Sunday, 9 October 2011 04:42 Written by Administrator Sunday, 9 October 2011 04:42
Molecular Biological and Immunological Techniques and Applications for Food Chemists
A guide to using molecular biology and immunological methods for the analysis of food
Many of the analytical problems that food chemists face in the lab cannot be solved by chemistry alone, and so analytical chemists are turning to molecular biology and immunology for alternative approaches. Molecular Biological and Immunological Techniques and Applications for Food Chemists comprehensively explains the most important molecular biology and immunology methods, and illustrates their application in food analysis.
Written by a distinguished group of experts, the coverage includes:
- Molecular Biological Methods—techniques explained, laboratory layout, PCR, real-time PCR, RFLP, SSCP, and sequencing
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Molecular Biology Applications—meat, genetically modified organisms (GMOs), food allergens, offal, and fish
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Immunological Methods—techniques explained and antibody-based detection methods
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Immunology Applications—animal speciation, international food allergen regulations (except Japanese), Japanese regulations and buckwheat allergen detection, egg allergen detection, soy allergen detection, milk allergen detection, gluten allergen detection, nut allergen detection, fish allergen detection, lupin allergen detection, mustard allergen detection, and celery allergen detection
Clearly written and consistently edited to provide information to a wide range of readers, Molecular Biological and Immunological Techniques and Applications for Food Chemists offers an up-to-date reference for food scientists in government and industry, policymakers, and graduate-level students of food science, technology, and engineering.
Note: CD-ROM/DVD and other supplementary materials are not included as part of eBook file.
List Price: $ 132.00
Price: $ 82.50
Tags: Applications, BIOLOGICAL, Chemists, food, Immunological, Molecular, Techniques | Posted under PCR Polymerase Chain Reaction Books | Comments Off
Last Updated on Tuesday, 26 July 2011 04:34 Written by Administrator Tuesday, 26 July 2011 04:34
To purchase the complete version of this video go to products.tafesa.edu.au Designed as a resource for teaching the practical techniques and applications of molecular biology, this DVD is well suited to educators and students in both the University and the Vocational Education and Training sectors. www.tafesa.edu.au To find out about Veterinary and Applied Science courses offered by TAFE SA go to www.tafesa.edu.au or call 1800 882 661.
Video Rating: 5 / 5
Tags: Introduction, Molecular, Techniques | Posted under PCR Polymerase Chain Reaction Reviews Videos | Comments Off
Last Updated on Tuesday, 19 July 2011 04:30 Written by Administrator Tuesday, 19 July 2011 04:30
Molecular Diagnostics: Techniques and Applications for the Clinical Laboratory
Advances in genomic and proteomic profiling of disease have transformed the field of molecular diagnostics, thus leading the way for a major revolution in clinical practice. While the range of tests for disease detection and staging is rapidly expanding, many physicians lack the knowledge required to determine which tests to order and how to interpret results. This handbook provides a complete guide to the use and interpretation of molecular testing in the clinical arena. No other available resource offers this emphasis, comprehensive scope, and practical utility in the clinical setting.
* Serves as the definitivereference for molecular pathologists worldwide
* Covers a variety of molecular techniques including next generation sequencing, tumor somatic cell genotyping, infectious and genetic disease tecting, and pharmacogenetics
* Discusses in the detail issues concerning quality assurance, regulation, ethics, and future directions for the science
List Price: $ 195.00
Price: $ 160.04
Tags: Applications, Clinical, diagnostics, Laboratory, Molecular, Techniques | Posted under PCR Polymerase Chain Reaction Books | Comments Off
Last Updated on Thursday, 16 June 2011 04:31 Written by Administrator Thursday, 16 June 2011 04:31
The random amplified polymorphic DNA (RAPD) assay and related techniques applied to genotoxicity and carcinogenesis studies: A critical review [An article ... Research-Reviews in Mutation Research]
This digital document is a journal article from Mutation Research-Reviews in Mutation Research, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
More than 9000 papers using the random amplified polymorphic DNA (RAPD) or related techniques (e.g. the arbitrarily primed polymerase chain reaction (AP-PCR)) have been published from 1990 to 2005. The RAPD method has been initially used to detect polymorphism in genetic mapping, taxonomy and phylogenetic studies and later in genotoxicity and carcinogenesis studies. Despite their extensive use, these techniques have also attracted some criticisms, mainly for lack of reproducibility. In the light of their widespread applications, the objectives of this review are to (1) identify the potential factors affecting the optimisation of the RAPD and AP-PCR assays, (2) critically describe and analyse these techniques in genotoxicity and carcinogenesis studies, (3) compare the RAPD assay with other well used methodologies, (4) further elucidate the impact of DNA damage and mutations on the RAPD profiles, and finally (5) provide some recommendations/guidelines to further improve the applications of the assays and to help the identification of the factors responsible for the RAPD changes. It is suggested that after proper optimisation, the RAPD is a reliable, sensitive and reproducible assay, has the potential to detect a wide range of DNA damage (e.g. DNA adducts, DNA breakage) as well as mutations (point mutations and large rearrangements) and therefore can be applied to genotoxicity and carcinogenesis studies. Nevertheless, the interpretation of the changes in RAPD profiles is difficult since many factors can affect the generation of RAPD profiles. It is therefore important that these factors are identified and taken into account while using these assays. On the other hand, further analyses of the relevant bands generated in RAPD profile allow not only to identify some of the molecular events implicated in the genomic instability but also to discover genes playing key roles, particularly in the initiation and development of malignancy. Finally, to elucidate the potential genotoxic effects of environmental contaminants, a powerful strategy could be firstly to use the RAPD assay as a screening method and secondly to apply more specific methods measuring for instance DNA adducts, gene mutations or cytogenetic effects. It is also envisaged that these assays (i.e. RAPD and related techniques), which reflect effects at whole genome level, would continue to complement the use of emerging technologies (e.g. microarrays which aim to quantify expression of individual genes).
List Price: $ 14.95
Price: $ 14.95
Tags: amplified, applied, article, Assay, carcinogenesis, critical, genotoxicity, Mutation, polymorphic, random, RAPD, related, research, ResearchReviews, Review, studies, Techniques | Posted under PCR Polymerase Chain Reaction Books | Comments Off