Posts Tagged ‘Protocols’
Last Updated on Wednesday, 5 October 2011 04:35 Written by Administrator Wednesday, 5 October 2011 04:35
Bioconjugation Protocols: Strategies and Methods (Methods in Molecular Biology)
Expert laboratorians update the classic bioconjugation methods and introduce valuable new techniques that go beyond pure biomedical applications to include elements from advanced organic synthesis, molecular biology, and materials science. These readily reproducible methods cover the preparation of protein conjugates using covalent and noncovalent conjugation, the synthesis of nucleic acid conjugates using a variety of labeling techniques, and approaches to semisynthetic conjugates of proteins. Additional chapters address the biofunctionalization of inorganic surfaces, including the on-chip synthesis of peptide nucleic acids to generate microarrays for the high-throughput analysis of RNA and DNA, gold nanaoparticles, and carbon nanotube probes for atomic force microscopy.
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Tags: Bioconjugation, Biology, Methods, Molecular, Protocols, Strategies | Posted under PCR Polymerase Chain Reaction Books | Comments Off
Last Updated on Thursday, 22 September 2011 04:33 Written by Administrator Thursday, 22 September 2011 04:33
Human Papillomaviruses: Methods and Protocols (Methods in Molecular Medicine)
Leading basic researchers and clinical scientists describe in detail a wide variety of established and cutting-edge techniques they have developed to study the lifecycle and biological properties of the human papillomavirus. The authors use these readily reproducible methods, ranging from PCR to propagation of HPV in vitro, to detect and type papillomavirus infections, study the papillomavirus lifecycle, and to produce and functionally analyze papillomavirus proteins. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
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Tags: Human, MEDICINE, Methods, Molecular, Papillomaviruses, Protocols | Posted under PCR Polymerase Chain Reaction Books | Comments Off
Last Updated on Monday, 5 September 2011 04:30 Written by Administrator Monday, 5 September 2011 04:30
In Vitro Mutagenesis Protocols (Methods in Molecular Biology)
Hands-on researchers with proven track records describe in stepwise fashion their advanced mutagenesis techniques. The contributors focus on improvements to conventional site-directed mutagenesis, including a chapter on chemical site-directed mutagenesis, PCR-based mutagenesis and the modifications that allow high throughput mutagenesis experiments, and mutagenesis based on gene disruption (both in vitro- and in situ-based). Additional methods are provided for in vitro gene evolution; for gene disruption based on recombination, transposon, and casette mutagenesis; and for facilitating the introduction of multiple mutations. Time-tested and highly practical, the protocols in In Vitro Mutagenesis Protocols, 2nd Edition offer today’s molecular biologists reliable and powerful techniques with which to illuminate the proteome.
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Tags: Biology, Methods, Molecular, mutagenesis, Protocols, vitro | Posted under PCR Polymerase Chain Reaction Books | Comments Off
Last Updated on Friday, 2 September 2011 04:33 Written by Administrator Friday, 2 September 2011 04:33
Embryonic Stem Cell Protocols: Volume I: Isolation and Characterization (Methods in Molecular Biology)
Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume one, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. The second volume, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. A companion CD provides electronic color versions of all illustrations in the book. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.
List Price: $ 179.00
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