Posts Tagged ‘cloning’
Last Updated on Saturday, 16 April 2011 08:34 Written by Administrator Saturday, 16 April 2011 08:34
Question by peeebs511: biology help? PCR, cloning, and stem cell research?
what is PCR (polymerase chain reaction), and what exactly is the process of cloning, and what are the difference between the two different types (reproductive and theraputic cloning)? and, why are stem cells use controversial?
Best answer:
Answer by Waldorf B
PCR is a technique used to amplify (make many copies) of DNA. Cloning is the making of an exact genetic replica of an organism. Reproductive cloning is cloning where an entire organism is cloned. Therapeutic cloning is the cloning of cells or tissues which would be useful for patients suffering from damaged cells or tissues of interest. The use of stem cells can be considered controversial as some may see it as playing God.
What do you think? Answer below!
Last Updated on Wednesday, 30 March 2011 04:37 Written by Administrator Wednesday, 30 March 2011 04:37
Cloning and analysis of non-specific cytotoxic cell receptor (NCCRP)-1 from common carp Cyprinus carpio L. [An article from: Comparative Biochemistry and Physiology, Part C]
This digital document is a journal article from Comparative Biochemistry and Physiology, Part C, published by Elsevier in 2005. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Nonspecific cytotoxic cell receptor protein (NCCRP-1) provides an important function in target cell recognition and activation of cytotoxicity. NCCRP-1 has been cloned from common carp Cyprinus carpio L. from fish barbel by EST analysis. The isolated gene is composed of 945 bp with a 79 bp 5′ UTR, 714 bp open reading frame and 152 bp 3′ UTR. The predicted NCCRP-1 gene is composed of 237 amino acid residues and its predicted signal peptide is 19 amino acid residues in length. This gene has conservation of all the related domains characteristic to the NCCRP-1 gene in fish. Phylogenetic and genomic analyses showed that carp NCCRP-1 was similar to other fish orthologues. The expression of NCCRP-1 gene was constitutive in both lymphoid and non-lymphoid tissues. Furthermore, by semi-quantitative RT-PCR studies, we showed that NCCRP-1 gene expression is increased in anterior kidney challenged with Aeromonas hydrophila.
List Price: $ 10.95
Price: $ 10.95
Tags: Analysis, article, Biochemistry, carp, carpio, Cell, cloning, common, Comparative, Cyprinus, cytotoxic, from, NCCRP1, nonspecific, Part, Physiology, Receptor | Posted under PCR Polymerase Chain Reaction Books | Comments Off
Last Updated on Friday, 25 February 2011 04:33 Written by Administrator Friday, 25 February 2011 04:33
Gene Cloning and Analysis by RT-PCR. [Subtitle]: (BioTechniques Molecular Laboratory Methids Series, 1.)
Price:
Tags: Analysis, BioTechniques, cloning, gene, Laboratory, Methids, Molecular, RTPCR, Series, Subtitle | Posted under PCR Polymerase Chain Reaction Books | Comments Off
Last Updated on Thursday, 10 February 2011 04:34 Written by Administrator Thursday, 10 February 2011 04:34
( www.abnova.com ) – The purpose of transformation is to introduce a foreign plasmid into bacteria and the bacteria will replicate the foreign plasmid along with their own DNA. Bacteria which are able to uptake DNA after a heat shock are called “competent”. More videos at Abnova http
Tags: cloning, Transformation | Posted under PCR Polymerase Chain Reaction Reviews Videos | 1 Comment