Posts Tagged ‘Amplification’
Last Updated on Friday, 23 September 2011 04:36 Written by Administrator Friday, 23 September 2011 04:36
Tags: AGING, Amplification, DQA1, effects, extraction, genotyping, loci, PCRbased, preservatives, storage, temperature, typing, Urinary, Using, Volume | Posted under PCR Polymerase Chain Reaction Books | Comments Off
Last Updated on Friday, 20 May 2011 08:33 Written by Administrator Friday, 20 May 2011 08:33
Question by mayukh: Regarding amplification of DNA..?
Regarding amplification of DNA initially polymerase chain reaction
was performed with a Klenow fragment of -
1) DNA Polymerase l of E.coli
2)DNA polymerase lll of Anthrax bacillus
3)RNA polymerase of E.coli
4)None of the above
Best answer:
Answer by Cindi B
The answer is 1.
I thought I knew the correct answer but looked it up just in case.
Klenow Fragment
Proteolytic product of E. Coli DNA polymerase I that has both polymerase and 3′ exonuclease activities and no 5′ exonuclease activity because fractionation of the digestion products removes the fragment with the 5′ exonuclease activity; a Klenow fragment with only DNA polymerase activity due to a mutation in the 3′ exonuclease sequence is also available.
What do you think? Answer below!
Tags: Amplification, DNA., Regarding | Posted under PCR Polymerase Chain Reaction Forum - Troubleshooting Questions & Answers | 1 Comment
Last Updated on Tuesday, 17 May 2011 04:31 Written by Administrator Tuesday, 17 May 2011 04:31
Question by Anthorien: What methods can be used to confirm a PCR product is not a non-specific amplification product.?
Best answer:
Answer by Theruchet
If you have a sequence of complementary DNA, RNA or a protein that is known to bind to the sequence of DNA that you were trying to polymerize then you could combine them and see if they do bind. Methods may vary to see if they do bind the DNA… here are a couple ideas:
1. the DNA, RNA or protein can be grown with a fluorescent or radioactice label attached. Then you would simply have to wash off your product (say run it through a gel filtration or affinity column) and see if the label remains.
2. If you know of a binding assay using a chromogenic substrate or a substrate with a known absorbance (that’s NOT the same as DNA absorbances…) you could perform this assay to see if the absorbance changes (absorbance usually changes by a measurable amount when a chromogenic substrate binds to another molecule).
This has been assuming that you’re polymerizing DNA. If you’re doing RNA just replace DNA with RNA everywhere I said it. I hope this is useful.
What do you think? Answer below!
Tags: Amplification, confirm, Methods, nonspecific, product, used | Posted under PCR Polymerase Chain Reaction Forum - Troubleshooting Questions & Answers | 1 Comment
Last Updated on Friday, 15 April 2011 12:31 Written by Administrator Friday, 15 April 2011 12:31
Question by Meghan M: The amplification of recombinant plasmids occurs by:?
a. the growth of the plasmid DNA.
b. DNA replication of plasmids outside a host cell.
c. the process of growth and division of the host cell.
e. the polymerase chain reaction.
Best answer:
Answer by Peter S
c. the process of growth and division of the host cell.
Know better? Leave your own answer in the comments!
Tags: Amplification, occurs, plasmids, RECOMBINANT | Posted under PCR Polymerase Chain Reaction Forum - Troubleshooting Questions & Answers | Comments Off