Polymerase chain reaction (PCR)
Polymerase chain reaction (PCR)
Last Updated on Saturday, 3 July 2010 08:49 Written by Administrator Saturday, 3 July 2010 08:49
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Nice video, Paul! (Matsudaira)…my daughter Carolina liked it a lot…best wishes, sandra
OMG its now soooo much clearer!!! thanks a bunch i looked at like 4 other vids and this on was the best (y)
this is a nice, clear video! excellent!
this made it so much more clearer
I GET IT!!!!!!! THANK YOU SO MUCH!
omg this saved me, this is so much easier to understand than anyother video
thx thx thx!!!
nice mic
I love you
it’s help me a lot….try pcr song….
thanksss
the best pcr video ever!!!!!!!!!
beautiful! this helped me a lot!
THANK YOU!
Thanks this helped me understand the process of PCR without having to kill myself with my textbook. This is going to help with my molecular biology midterm which is in a few hours lol.
oh dis iz sooo gud!!
Thanks for posting this.
specific machine? yes there are, the thermal cycling machine can be bought from Thermo Fisher Scientific.
As for the primers, they are synthesis chemically. Find out how to make a short oligonucleotide from google. They are basically of the same principle.
The ones that I use in lab are purchased from a company which can produce it in commercial quantity. They even give the exact sewuance of the primers in the manual…
Are there specific machines that automate the process? Much like a pcr machine?
Is the required DNA code for the primer input as code manually (like typed in from a keyboard), or is the primer synthesised chemically from a prior existing DNA segment (ie: the required DNA must already exist).
If the latter – how do they target and handle this ONE singular DNA segment? I mean, it’s a single, rather short, small DNA segment, it’s not like they can look for it & pick it up with tongs right?
You are right. The primers are design specifically. In one strand of DNA, there are many genes, thus we need to design the primers such that only the target sequence is amplified, not others.
There are many papers discussing how to design the primers. Usually, you have to know the actual sequence before you can design ones.
Yesh! now i feel more confident about my exam
This is awesome. Thank you!
excellent video..must say…thnx pench
brilliant.
What? Of course they were “made”! They didn’t just pop into existance all on their own.
Because they seem to be one of the few “designer parts” in the reaction. Everything else seems natural, but the primers will ONLY attach to the specific DNA sequence the experimenter is interested in. So i’m curious how the experimenter makes these primers.
They seem to be one of the few man-made elements in the reaction.
they are not “made”, they were in the mixture from the beginning
Thank you so much for this…
You have probably saved me hours of fruitless research and now I think I understand PCR pretty well.
By the way, I found out something while I was researching PCR.
There are other polymerases apart from taq (higher error rate because it can not proofread) that have a proofreading ability and can still withstand the higher temperatures (such as vent and pfu polymerases).