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unboxing qPCR part 6: Amplify Sample with The StepOnePlus™ Real Time System by Life Technologies

The StepOnePlus™ Real-Time PCR System is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users. The StepOnePlus™ Real-Time PCR System can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Utilizing robust LED based 4-color optical recording, the StepOnePlus™ Real-Time PCR System is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications. Transcript: Alright. Let’s recap a typical real-time PCR or qPCR experiment in a plate. What you will require is the actual plate, the plastics and the plastic holder. The optical adhesive cover and the optical adhesive sealer. In addition for the reagents, if you’re doing a Taqman experiment, you will require the Taqman fast advance master mix. TaqMan gene expression assay. Sybr experiments will require the sybr ring master mix, and, 2 primers, which I have designated, primer 1 and primer 2. P 1 and P 2. Also. When we fire your CDNA sample and finally some water. These pipe [indiscernible] are to show you that you essentially pipette the reagents into the plate. You then seal the plate. This is the final product, which we then run on the instrument and in this case the Step One Plus. And now I’m walking over to the Step One Plus. I’m going to load my plate in the instrument simply by pulling open the door. And setting the plate, The A goes up in the top as usual …