does buffer pH change during PCR?
does buffer pH change during PCR?
Last Updated on Sunday, 12 June 2011 12:31 Written by Administrator Sunday, 12 June 2011 12:31
Question by 123: does buffer pH change during PCR?
PCR=Polymerase chain reaction
Best answer:
Answer by usc916
The question doesn’t really make much sense. The purpose of a buffer is to keep the pH the same.
My guess for PCR is that the pH stays the same. DNA can only stay natured in specific pH conditions
Know better? Leave your own answer in the comments!
pH is critical to success of PCR and can be manipulated to provide ideal conditions for various target lengths.